Human brucellosis mimicking axial spondyloarthritis: a challenge for rheumatologists when applying the 2009 ASAS criteria / 华中科技大学学报（医学）（英德文版）

Although the development of the 2009 SpA classification criteria by Assessment of SpondyloArthritis international Society (ASAS) represents an important step towards a better definition of the early disease stage particularly in axial spondyloarthritis (axSpA), the specificity of the criteria has been criticized these days. As the commonest zoonotic infection worldwide, human brucellosis can mimic a large number of diseases, including SpA. This study was performed to determine the frequency of rheumatologic manifestations in patients with brucellosis and the chance of misdiagnosing them as having axSpA in central China. The results showed that clinical manifestations of axSpA could be observed in brucellosis. Over half of patients had back pain, and one fifth of the patients with back pain were less than 45 years old at onset and had the symptom for more than 3 months. Two young males were falsely classified as suffering from axSpA according to the ASAS criteria, and one with MRI proved sacroiliitis was once given Etanercept for treatment. Therefore, differential diagnosis including human brucellosis should always be kept in mind when applying the ASAS criteria, even in traditionally non-endemic areas.

Purification technology of Lonicerae Japonicae Flos materiomic based on its fingerprint / 中草药

Objective: To optimize the technological parameters in the purification process of Lonicerae Japonicae Flos (LJF) materiomic with HPLC fingerprint technique. Methods: Using the established HPLC fingerprint of LJF, the macroporous resin type, adsorption conditions, and elution requirements were optimized using the similarity and recovery rate of fingerprints of eight components (neochlorogenic acid, chlorogenic acid, cryptochlorogenin acid, vogeloside, luteoloside, isochlorogenic acid A, isochlorogenic acid B, and isochlorogenic acid C) as indexes. Results: XDA-8 type resin possessed the highest recovery rates of eight components. The optimized purification process was as follows: The sample concentration was 0.25 g/mL, the ratio of sample to resin was 0.9 g/g, pH value was 3.5, and 40% ethanol (four times of resin volume) and 80% ethanol (four times of resin volume) were used as eluant, respectively. After being purified, the recovery rates of eight components were 78%-96%, and the similarity of fingerprint was higher than 0.98. These components could get balanced recovery. Conclusion: Based on HPLC fingerprint technique, the optimized purification process of LJF materiomic with macroporous resin can ensure that the main components are consistent with the crude extract of LJF.

Simultaneous quantitative analysis of four lignanoids in Schisandra chinensis by quantitative analysis of multi-components by single marker / 药学学报

The aim of the study is to establish a new method of quality evaluation and validate its feasibilities by the simultaneous quantitative assay of four lignanoids in Schisandra chinensis. A new quality evaluation method, quantitative analysis of multi-components by single marker (QAMS), was established and validated with Schisandra chinensis. Four main lignanoids, schisandrin, schisantherin A, deoxyschizandrin and gamma-schizandrin, were selected as analytes and schisandrin as internal reference substance to evaluate the quality. Their contents in 13 different batches of samples, collected from different bathes, were determined by both external standard method and QAMS. The method was evaluated by comparison of the quantitative results between external standard method and QAMS. No significant differences were found in the quantitative results of four lignanoids in 13 batches of S. chinensis determined by external standard method and QAMS. QAMS is feasible for determination of four lignanoids simultaneously when some authentic standard substances were unavailable, and the developed method can be used for quality control of S. chinensis.

Heterologous functional expression system for odorant receptors.

Heterologous functional expression system for odorant receptors (ORs) is essential for investigating the structure-activity relationship (SAR) of various ligands. Different systems that coexpressed ORs with different G-protein alpha subunits (Galpha) demonstrated inconsistent effects on weak agonists and antagonists, but retained original relative sensitivities to potent agonists. In order to maintain the binding specificity of Galpha to ORs, we constructed a chimeric Galpha(15_olf), which contained the Galpha(15) sequence with the conserved C-terminal region of Galpha(olf). The Ca(2+) responses of the HEK293 cells that coexpressed OR-S6 with Galpha(15_olf) were more robust and reproducible compared to those of cells that coexpressed OR-S6 with Galpha(15). Furthermore, Galpha(15) sometimes induced unstable Ca(2+) responses that limited the accuracy of quantitative comparison of peak responses. Our results showed that a heterologous expression system that coexpressed ORs with Galpha(15_olf) and receptor transporting proteins was suitable for SAR analysis of various ligands.

Expression of CD147, matrix metalloproteinases and transforming growth factor beta1 in breast cancer / 中华病理学杂志

<p><b>OBJECTIVE</b>To investigate the expression of CD147, matrix metalloproteinase (MMP)-2, MMP-9, Transforming growth factor (TGFbeta1) and TGFbetaRI proteins and their relationships to breast cancer.</p><p><b>METHODS</b>The expression of CD147, MMP-2, MMP-9, TGFbeta1 and TGFbetaRI proteins was examined on tissue chips containing 160 cases of breast carcinomas by S-P immunohistochemical method.</p><p><b>RESULTS</b>The positive rates of CD147, MMP-2, MMP-9, TGFbeta1 and TGFbetaRI proteins were 87.5% (140/160), 96.9% (155/160), 95.0% (152/160), 73.7% (118/160) and 60.6% (97/160), respectively. The expression of CD147 was positively correlated with axillary lymph node metastasis, TNM staging and HER2 over expression (P < 0.01, P < 0.05 and P < 0.01, respectively), and inversely correlated with PR expression (P < 0.05). The patients' relapse-free survival was shorter in TGFbeta1-positive group than in TGFbeta1 negative group (P < 0.05). Both the expression of MMP-2 and MMP-9 were positively correlated with CD147 expression; and both the expression of TGFbeta1 and TGFbetaRI were positively correlated with CD147 expression (P < 0.01 and P < 0.05, respectively).</p><p><b>CONCLUSION</b>The expression of CD147 is considered closely correlated with tumor invasion, metastasis and prognosis in breast cancer, and has also a close correlation with MMP-2, MMP-9, TGFbeta1 and TGFbetaRI expression.</p>

A case of deep mycosis caused by Rhizomucor chlamydosporus / 中华皮肤科杂志

Objective To report a case of deep mycosis caused by Rhizomucor chlamydosporus. Methods Medical history,histopathology and laboratory examination were investigated,and fungal identifi- cation by microscopy and culture as well in the patient.Results The patient,a 41-year-old male,initially presented with mild-tender and progressively aggravating masses on the right glutea,both groins,and back of the head of pancreas.Later,ulcer,necrosis,and black crusts developed at the primary lesions accompanied with nausea,vomitting and dysfunction of liver.Pathological examination revealed a chronic granuloma- tous inflammation in the dermis and subcutaneous tissue;and branching,nonseptate and broad hyphae in multinuclear giant cells,tissue spaces and blood vessel lumens,and,few PAS-positive septate hyphae as well as basophilic chlamydospores located in multinuclear giant cells.The isolate was identified as R. chlamydosporus.Conclusions The case of deep mycosis caused by R.chlamydosporus began with invasive granuloma,followed by necrotic ulcer,with condition aggravating rapidly,and the patient finally died of se- rious cachexia.

Autologous peripheral blood stem cell transplantation for systemic lupus erythematosns / 中华风湿病学杂志

20?10~9/L.The proteinuria decreased or disappeared.The antinuclear antibody decreased or became negative.The level of complement was increased.The following complications were ob- served:septicemia in 2 patients,cytomegalovirus infection in 2 patients,renal toxicity in 1 patient,acute left heart failure in 3 patients and cardiac arrhythmia in 3 patients.There was no transplantation related mortality. Conclusion APBSCT may improve the disease activity and the immunological markers in SLE.It is a valid therapy for refractory SLE,but the long-term effects need to be observed.